Phenotype Image Detail

Image

Caption

(A) Apoptosis in Tdrd5^tm1Sait/Tdrd5^tm1Sait (-/-) testes. Wild-type (a) and mutant (b) testes stained with anti-activated Caspase3 antibody (brown) counterstained with hematoxylin. Magnified images of mutant seminiferous tubules at stages I (c), II and III (d), IV-VI (e), VII (f), IX (g), X (h), XI (i), and XII (j) are shown. (B) Nuclear-spread analysis of the meiotic prophase in wild-type (left) and mutant (right) testes. Meiotic spermatocytes were staged (leptotene, zygotene and pachytene) based on the nuclear morphology (Hoechst) and the distribution pattern of SCP3 and gammaH2AX. The bright spots detected by gammaH2AX in the pachytene spermatocytes are XY bodies. (C) Total counts of the indicated cells from hematoxylin and eosin-stained sections of wild-type (white) and mutant (black) testes. PL, preleptotene; L, leptotene ; Z, zygotene; P, pachytene; D, diplotene spermatocytes; RS, round spermatids. (D) Percentage (the average with SD; error bars) of the indicated cells in the surface spreads of wild-type (white) and mutant (black) testes. (E) Acrosome visualized by PNA (red) and Golgi58K (green) staining of wild-type (left, white arrowhead, stage V) and mutant (right, green arrowheads, stage V-VII) round spermatids, counter-stained with Hoechst (white). (F) Histology of a mutant seminiferous tubule (left, adult; right, 4 week old) with a severe phenotype stained by hematoxylin and eosin. Bars: (A, a and b) 50 um: (A, c-j) 10 um; (B and E) 10 um; (F) 20 um.

Copyright

This image is from Yabuta Y, J Cell Biol 2011 Mar 7;192(5):781-95, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:170319

Associated Alleles

Symbol	Name
Tdrd5^tm1Sait	tudor domain containing 5; targeted mutation 1, Mitinori Saitou

Associated Genotypes

Allelic Composition	Genetic Background
Tdrd5^tm1Sait/Tdrd5^tm1Sait	involves: C57BL/6 * CBA