Phenotype Image Detail

Image

Caption

(A) Smaller testes in Mcph1^tm1.2Kali/Mcph1^tm1.2Kali (BRIT1^-/-) mice. Shown here are the testes from the indicated mice at P28. Scale bar, 5 mm. (B,C) Thinner seminiferous epithelia in mutant mice. Testes sections from wild-type (BRIT1^+/+, B) and mutant (C) mice at P28 were stained with hematoxylin-eosin (H&E). Arrows, the seminiferous tubules; L, lumen of the seminiferous tubules. Scale bar, 50 um. (D) Mitosis was not defective in mutant spermatogonia and Sertoli cells. Testes at P7 were either stained with H&E or double-stained with anti-Tra98 and anti-Sox9 antibodies using immunofluorescent staining. Scale bar, 50 um. (E) Meiotic dysregulation in mutant spermatocytes. H&E staining was performed in testis sections from wild-type and mutant littermates at P14, P21, and P56. Black arrows: spermatocytes at leptotene/zygotene (a,b,d,f); white arrows: spermatocytes at diplotene (c); green arrows: elongate spermatids (e). Scale bar, 50 um.

Copyright

This image is from Liang Y, PLoS Genet 2010 Jan;6(1):e1000826, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:156371

Associated Alleles

Symbol	Name
Mcph1^tm1.2Kali	microcephaly, primary autosomal recessive 1; targeted mutation 1.2, Kaiyi Li

Associated Genotypes

Allelic Composition	Genetic Background
Mcph1^tm1.2Kali/Mcph1^tm1.2Kali	involves: 129S7/SvEvBrd * C57BL/6J