Phenotype Image Detail

Image

Caption

Day 42 caput epididymides were Bouin's-fixed, PAS-stained and hematoxylin-counter-stained to visualize the glycoproteins (pink) and nuclei (blue) and analyzed by light microscopy using a 40x objective lens. (A) Wild-type (+/+) epididymides contained a columnar epithelial layer (E) with a smooth actin layer (arrow) beneath the long PAS-positive microvilli that extend into the lumen. Mature spermatozoa (S) were present in the lumen. (B) The Ehd1^tm1.2Haba/Ehd1^tm1.2Haba (-/-) epididymides contained a few sloughed round spermatids (Spt) and spermtocytes (Spc). Scale bar = 20 um.

Copyright

This image is from Rainey MA, BMC Dev Biol 2010;10():37, an open-access article, licensee BioMed Central Ltd. J:160452

Associated Alleles

Symbol	Name
Ehd1^tm1.2Haba	EH-domain containing 1; targeted mutation 1.2, Hamid Band

Associated Genotypes

Allelic Composition	Genetic Background
Ehd1^tm1.2Haba/Ehd1^tm1.2Haba	involves: 129P2/OlaHsd * C57BL/6J * FVB/N