Phenotype Image Detail

Image

Caption

(A-F) Kluver-Barrera Luxol fast blue staining in the cortex, corpus callosum, and internal capsula of 20-month-old mice expressing wild-type Notch3 (TgNotch3^WT) and mice expressing a mutant Notch3, Tg(Notch3*R169C)88Bbb/0 (TgNotch3^R169C) mice revealing vacuolization in white matter bundles as well as pallor and disorganization of the myelin in mutant mice. (G) Quantification of the vacuole area in the cortex, corpus callosum, fimbria of hippocampus, and anterior commissural (Ant. Comm.) of 20-month-old Tg(Notch3*R169C)88Bbb/0 mutants compared with TgNotch3^WT mice (n = 3-4 mice per group). (H-M) Glial fibrillary acidic protein (GFAP) staining in the cortex, corpus callosum, and internal capsula of 20-month-old TgNotch3^WT and Tg(Notch3*R169C)88Bbb/0 mice showing astrogliosis in the white matter bundles of Tg(Notch3*R169C)88Bbb/0 mutant mice. (N) Quantification of astrogliosis in the indicated structures of 20-month-old Tg(Notch3*R169C)88Bbb/0 mutant mice compared with TgNotch3^WT mice (n = 4 mice per group). Scale bars: 70 um.

Copyright

This image is from Joutel A, J Clin Invest 2010 Feb 1;120(2):433-45 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:156682

Associated Alleles

Symbol	Name
Tg(Notch3*R169C)88Bbb	transgene insertion 88, Norbert Hubner

Associated Genotypes

Allelic Composition	Genetic Background
Tg(Notch3*R169C)88Bbb/0	involves: FVB/N