Phenotype Image Detail

Image

Caption

(A) Masson's trichrome staining showing intact pial artery (upper panels) and small artery (lower panels) of 20-month-old Tg(Notch3*R169C)88Bbb/0 (TgNotch3^R169C) mice. (B) Labeling for smooth muscle myosin heavy chain revealing a continuous rim of smooth muscle cells in pial and small artery of 20-month-old Tg(Notch3*R169C)88Bbb/0 mutant mice. (C) Double labeling for Notch3 extracellular domain (Notch3^ECD) (5E1, red) and collagen IV (green) of capillaries in the cortext and the corpus callosum of 12-month-old Tg(Notch3*R169C)88Bbb/0 mutant mice showed robust aggregation of Notch3^ECD in both capillaries. (D) Mean total capillary length (mm of CD31+ structures per mm²) in the cortex of 12- and 20-month-old Tg(Notch3*R169C)88Bbb/0 mice and the corpus callosum of 6- 12-, and 20-month-old Tg(Notch3*R169C)88Bbb/0 mice compared with age-matched TgNotch3^WT mice (expressing wild-type Notch3) showed progressive age-related reduction in capillary density in the white matter of Tg(Notch3*R169C)88Bbb/0 mutant mice (n = 4-5 mice per group). Scale bars: 25 um.

Copyright

This image is from Joutel A, J Clin Invest 2010 Feb 1;120(2):433-45 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:156682

Associated Alleles

Symbol	Name
Tg(Notch3*R169C)88Bbb	transgene insertion 88, Norbert Hubner

Associated Genotypes

Allelic Composition	Genetic Background
Tg(Notch3*R169C)88Bbb/0	involves: FVB/N