Phenotype Image Detail

Image

Caption

Vascular phenotype of Adam17^tm1Imx/Adam17^tm1Imx embryos. Whole-mount E14.5 embryos were stained with CD31 (PECAM) antibodies to characterize their vascular morphology. Panel A shows the vasculature of an intact Adam17^tm1Imx/Adam17⁺ (Adam17^deltaZn/+) embryo. Boxed area indicates the location of the region shown at higher magnification in panel B after dissection to allow flat mounting to view the cranial vessels. Similar views from heads of wild-type (Adam17^+/+) (C) Adam17^tm1Imx/Adam17^tm1Imx (Adam17^{deltaZn/deltaZn}) (D) embryos. Wild-type patterning of the branches is severely disrupted in the homozygous mutant embryonic heads. Vessels numbered 1-4 are stunted and less branched in the mutant embryos. Although the distance between vessel 2 and the branch leading up to vessels 3 and 4 seems increased in the mutant head shown (red bar), this distance was variable in wild-type heads. The asterisk marks a vessel damaged during dissection. Scale bars are 200 um.

Copyright

This image is from Canault M, PLoS One 2010;5(10):e13433, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:166217

Associated Alleles

Symbol	Name
Adam17^tm1Imx	a disintegrin and metallopeptidase domain 17; targeted mutation 1, Immunex

Associated Genotypes

Allelic Composition	Genetic Background
Adam17^tm1Imx/Adam17^tm1Imx	involves: 129 * C57BL/6J