Phenotype Image Detail

Image

Caption

(A) Left panel, representative testis from 12 month old wild-type and Alkbh1^tm1Klng/Alkbh1^tm1Klng (Alkbh1-/-) males. Right panel, average testis weight (mg) from 12 week old wild-type (110.6 +/- 10.4 mg, n = 12) and mutant (85.6 +/- 8.4 mg, n = 20) males, and 12 month old wild-type (114.4 +/- 8.9 mg, n = 6) and mutant (74.3 +/- 12.1 mg, n = 24 ) males (B) TUNEL staining (middle panel) and DAPI staining (left panel) of testis sections from 9 month old wild-type and mutant mice, showing apoptosis in mutant round (arrowhead) and elongating (arrow) spermatids, and in degenerated germ cells in the subbasal layers of the tubules (middle and right panel). Closer view of one apoptotic elongating spermatid is shown in the lower panels. (C) Double immunostaining of XY-bodies in mid-pachytene cells. Testicular cells from 12 month old wild-type and mutant males were spread and staind with two different markers against XY-bodies. MacroH2A (green), FK2 (red) and DAPI (blue). Magnifications: B and C, x20.

Copyright

This image is from Nordstrand LM, PLoS One 2010;5(11):e13827, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:166841

Associated Alleles

Symbol	Name
Alkbh1^tm1Klng	alkB homolog 1, histone H2A dioxygenase; targeted mutation 1, Arne Klungland

Associated Genotypes

Allelic Composition	Genetic Background
Alkbh1^tm1Klng/Alkbh1^tm1Klng	involves: 129X1/SvJ * C57BL/6