Phenotype Image Detail

Image

Caption

(A) PFK activity was determined in heart extracts. Basal PFK activity in wild-type (WT) mice was 27.4 +/-5.4 U/g tissue. (B,C) Glucose-6-phosphate (B) and glycogen concentrations (C) were determined in heart perchloric extracts from 2 month old wild-type and Pfkm^tm1Fbos/Pfkm^tm1Fbos (Pfkm-/-) mice. Results are mean +/- SEM of five mice per group. *P<0.05, **P<0.01 vs. wild-type. (D) Transmission electron microscopic analysis of cardiac muscle. Arrows show glycogen storage. Scale bar 2 um. (E,F) One year old mutant mice develop cardiac hypertrophy, evidenced by hematoxylin-eosin staining of heart sections (scale bar 1 mm) (E) and cardiomegaly (F). (G) Longitudinal sections of heart from 3 month old mice stained with Masson trichromic reagent (scale bar 1 mm). Inset shows septum sections (scale bar 50 um).

Copyright

This image is from Garcia M, PLoS Genet 2009 Aug;5(8):e1000615, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:152153

Associated Alleles

Symbol	Name
Pfkm^tm1Fbos	phosphofructokinase, muscle; targeted mutation 1, Fatima Bosch

Associated Genotypes

Allelic Composition	Genetic Background
Pfkm^tm1Fbos/Pfkm^tm1Fbos	involves: 129S6/SvEvTac * C57BL/6J