Phenotype Image Detail

Image

Caption

Growth of Nipbl^{Gt(RRS564)Byg}/Nipbl⁺ mice. (A,B) Photographs of pairs of newborn (A) and 4 week old (B) wild-type (+/+) and Nipbl^{Gt(RRS564)Byg}/Nipbl⁺ (+/-) littermates, illustrating obvious growth retardation. (C-E) Growth curves for the N0 (C), N1 (D), and N2 (E) generations, starting at weaning age. Data are pooled by genotype and sex (N0 wild-type: 15 males and 7 females; N0 mutant: 12 males and 7 females; N1 wild-type 39 males and 28 females; N1 mutant: 23 males and 17 females; N2 wild-type: 72 males and 38 females, N2 mutant: 39 males and 23 females): filled squares = male, open circles = female; red symbols = mutant, black symbols = wild-type; error bars = SEM. Mutant mice of both sexes are smaller, and exhibit less weight gain after maturity. (F) Growth from birth through 6 weeks. Wild-type data (black symbols and lines) are pooled by sex: filled squares = male, open circles = female (data from 28 females and 26 males. error bars = SEM). The records of individual mutant mice (N2 and N3 generation) are shown as connected lines without symbols. Red lines represent individual animals that survived at least 6 weeks (dashed = female; solid = male). Blue lines are mutant mice that died before weaning. Green lines represent additional mice that died before weaning for which genotype could not be established (due to cannibalism or tissue decomposition). The inset magnifies the pre-weaning interval (1 to 22 days). Whereas wild-type mice grow at a nearly linear rate during the first two weeks of life, the data show that growth of most mutant mice arrests between postnatal days 5 and 15, followed, in about a third of cases, by death several days later. Several of the mutants that stopped growing and lost weight after day 12, however, were able to resume rapid weight gain immediately after weaning.

Copyright

This image is from Kawauchi S, PLoS Genet 2009 Sep;5(9):e1000650, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:154117

Associated Alleles

Symbol	Name
Nipbl^Gt(RRS564)Byg	NIPBL cohesin loading factor; gene trap RRS564, BayGenomics

Associated Genotypes

Allelic Composition	Genetic Background
Nipbl^Gt(RRS564)Byg/Nipbl^+	involves: 129P2/OlaHsd * CD-1
Nipbl^Gt(RRS564)Byg/Nipbl^+	involves: 129P2/OlaHsd