Phenotype Image Detail

Image

Caption

Histopathology of thymus from wild-type, Sgpl1^{Gt(OST58278)Lex}/Sgpl1^{Gt(OST58278)Lex}, Sgpl1^{tm1(SGPL1)Lex}/Sgpl1^{tm1(SGPL1)Lex} and Sgpl1^{tm1(SGPL1)Lex}/Sgpl1^{Gt(OST58278)Lex} mice. (A) Wild-type thymus. A broad thymic cortex is densely packed with thymic lymphocytes (lower panel) (B) Sgpl1^{Gt(OST58278)Lex}/Sgpl1^{Gt(OST58278)Lex} (S1PL^-/-) thymus: The hypoplastic cortex is very thin and hypocellular due to severe cortical lymphoid depletion and apoptosis. At higher magnification (lower panel) the remaining cells in the thymic cortex include vacuolated stromal/epithelial cells and smaller numbers of granulocytes, macrophages, lymphocytes, and apoptotic cells. In contrast, the markedly expanded and hypercellular thymic medulla of mutant mice contains numerous mature lymphocytes as well as vacuolated stromal/epithelial cells, macrophages, and histiocytes (lower panel). (C) Sgpl1^{tm1(SGPL1)Lex}/Sgpl1^{tm1(SGPL1)Lex} (S1PL^H/H) thymus. The cortex contains minimally decreased numbers of lymphocytes while there is a minimal expansion of the medulla by increased numbers of lymphocytes (D) Sgpl1^{tm1(SGPL1)Lex}/Sgpl1^{Gt(OST58278)Lex} (S1PL^H/-) thymus. Thinning of the thymic cortex is accompanied by mild expansion of the medulla due primarily to increased numbers of lymphocytes (lower panel). H&E stain.

Copyright

This image is from Vogel P, PLoS ONE 2009;4(1):e4112, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:145159

Associated Alleles

Symbol	Name
Sgpl1^Gt(OST58278)Lex	sphingosine phosphate lyase 1; gene trap OST58278, Lexicon Genetics
Sgpl1^tm1(SGPL1)Lex	sphingosine phosphate lyase 1; targeted mutation 1, Lexicon Genetics

Associated Genotypes

Allelic Composition	Genetic Background
Sgpl1^Gt(OST58278)Lex/Sgpl1^Gt(OST58278)Lex	involves: 129S5/SvEvBrd * C57BL/6J
Sgpl1^tm1(SGPL1)Lex/Sgpl1^tm1(SGPL1)Lex	involves: 129S4/SvJae * 129S5/SvEvBrd * C57BL/6J
Sgpl1^Gt(OST58278)Lex/Sgpl1^tm1(SGPL1)Lex	involves: 129S5/SvEvBrd * C57BL/6J