Phenotype Image Detail

Image

Caption

Histopathology of mesenteric lymph nodes and intestinal lamina propria from wild-type, Sgpl1^{Gt(OST58278)Lex}/Sgpl1^{Gt(OST58278)Lex}, Sgpl1^{tm1(SGPL1)Lex}/Sgpl1^{tm1(SGPL1)Lex} and Sgpl1^{tm1(SGPL1)Lex}/Sgpl1^{Gt(OST58278)Lex} mice. (A) Wild-type (S1PL^+/+) lymph node: Paracortical zones are filled primarily with lymphocytes (B) Sgpl1^{Gt(OST58278)Lex}Sgpl1^{Gt(OST58278)Lex} (S1PL^-/-) lymph node: Small B cell lymphoid follicles appear as small semicircular caps overlying an expanded but relatively hypocellular paracortical zones. At higher magnification (lower panel), the paracortex contains markedly increased numbers of granulocytes and macrophages (some vacuolated and containing karyorrhectic debris), mixed with stromal cells and plasma cells. (C) Sgpl1^{tm1(SGPL1)Lex}/ Sgpl1^{tm1(SGPL1)Lex} (S1PL^H/H) lymph node: There is marked expansion of paracortical zones. At higher expansion, this expansion is due primarily to sequestered lymphocytes. (D) Sgpl1^{tm1(SGPL1)Lex}/Sgpl1^{Gt(OST58278)Lex} (S1PL^H/-) lymph node: Similarly, the marked expansion of paracortical zones is due primarily to sequestered lymphocytes (lower panel). (E) Wild-type lamina propria: Lymphocytes and plasma cells predominate in the lamina propria of wild-type mice. (F) Sgpl1^{Gt(OST58278)Lex}Sgpl1^{Gt(OST58278)Lex} lamina propria: There is a marked depletion of lymphocytes and plasma cells and increased numbers of granulocytes (arrows) in the intestinal lamina propria. (G) Sgpl1^{tm1(SGPL1)Lex}/Sgpl1^{tm1(SGPL1)Lex} lamina propria: Essentially normal cell populations are present. (H) Sgpl1^{tm1(SGPL1)Lex}/Sgpl1^{Gt(OST58278)Lex} lamina propria: Essentially normal cell populations are present. H&E stain.

Copyright

This image is from Vogel P, PLoS ONE 2009;4(1):e4112, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:145159

Associated Alleles

Symbol	Name
Sgpl1^Gt(OST58278)Lex	sphingosine phosphate lyase 1; gene trap OST58278, Lexicon Genetics
Sgpl1^tm1(SGPL1)Lex	sphingosine phosphate lyase 1; targeted mutation 1, Lexicon Genetics

Associated Genotypes

Allelic Composition	Genetic Background
Sgpl1^Gt(OST58278)Lex/Sgpl1^Gt(OST58278)Lex	involves: 129S5/SvEvBrd * C57BL/6J
Sgpl1^tm1(SGPL1)Lex/Sgpl1^tm1(SGPL1)Lex	involves: 129S4/SvJae * 129S5/SvEvBrd * C57BL/6J
Sgpl1^Gt(OST58278)Lex/Sgpl1^tm1(SGPL1)Lex	involves: 129S5/SvEvBrd * C57BL/6J