Phenotype Image Detail

Image

Caption

(A and B) Blood glucose (A) and plasma insulin (B) concentrations in wild-type (WT) (n = 7), Cebpb^tm1.1Maka/Cebpb^tm1.1Maka Tg(Ins2-cre)23Herr/0 (Beta Cebpb^-/-) (n = 9), Ins2^Akita/Ins2^Akita (Akita) (n = 7), and Ins2^Akita/Ins2^Akita Cebpb^tm1.1Maka/Cebpb^tm1.1Maka Tg(Ins2-cre)23Herr/0 (n = 14) mice in the fed state and at the indicated ages. Data are mean +/- SEM. *P < 0.05, ** P < 0.01, Ins2^Akita/Ins2^Akita Cebpb^tm1.1Maka/Cebpb^tm1.1Maka Tg(Ins2-cre)23Herr/0 versus Ins2^Akita/Ins2^Akita. (C) Pancreatic sections from 8-week-old wild-type (WT), Cebpb^tm1.1Maka/Cebpb^tm1.1Maka Tg(Ins2-cre)23Herr/0 (Beta Cebpb^-/-), Ins2^Akita/Ins2^Akita (Akita), and Ins2^Akita/Ins2^Akita Cebpb^tm1.1Maka/Cebpb^tm1.1Maka Tg(Ins2-cre)23Herr/0 (Akita;Beta Cebpb^-/-) mice were stained with antibodies to insulin (red) and to glucagon (green). Scale bars: 100 um. (D) Quantitation of Beta cell mass in 8-week-old WT, Cebpb^tm1.1Maka/Cebpb^tm1.1Maka Tg(Ins2-cre)23Herr/0, Ins2^Akita/Ins2^Akita, and Ins2^Akita/Ins2^Akita Cebpb^tm1.1Maka/Cebpb^tm1.1Maka Tg(Ins2-cre)23Herr/0 mice. Data are mean +/- SEM from 5 mice per genotype. *P <0.05. (E) Insulin content of the pancreas of WT, Cebpb^tm1.1Maka/Cebpb^tm1.1Maka Tg(Ins2-cre)23Herr/0, Ins2^Akita/Ins2^Akita, and Ins2^Akita/Ins2^Akita Cebpb^tm1.1Maka/Cebpb^tm1.1Maka Tg(Ins2-cre)23Herr/0 mice at 12 weeks of age. Data are mean +/- SEM from 4 mice per genotype. *P <0.05.

Copyright

This image is from Matsuda T, J Clin Invest 2010 Jan;120(1):115-26 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:156725

Associated Alleles

Symbol	Name
Cebpb^tm1.1Maka	CCAAT/enhancer binding protein beta; targeted mutation 1.1, Masato Kasuga
Ins2^Akita	insulin II; Akita
Tg(Ins2-cre)23Herr	transgene insertion 23, Pedro Luis Herrera

Associated Genotypes

Allelic Composition	Genetic Background
Cebpb^tm1.1Maka/Cebpb^tm1.1Maka Ins2^Akita/Ins2^Akita Tg(Ins2-cre)23Herr/0	involves: 129P2/OlaHsd * C57BL/6J * C57BL/6NSlc * CBA/J
Ins2^Akita/Ins2^Akita	involves: C57BL/6NSlc