Phenotype Image Detail

Image

Caption

Cardiac fibroblasts are essential for the protective response elicited by severe pressure overload. (A-H) Klf5^tm2.1Rng/Klf5^tm2.1Rng (Klf5^fl/fl) and Klf5^tm2.1Rng/Klf5^tm2.1Rng Tg(Postn-cre)1Sjc/0 (Klf5^fl/fl;Postn-Cre) mice were subjected to high-intensity transverse aortic constriction (HI-TAC) or sham operation. (A) Kaplan-Meier survival analysis of Klf5^tm2.1Rng/Klf5^tm2.1Rng (n = 16) and Klf5^tm2.1Rng/Klf5^tm2.1Rng Tg(Postn-cre)1Sjc/0 (n = 10) mice after HI-TAC. *P < 0.05 versus Klf5^tm2.1Rng/Klf5^tm2.1Rng. (B) Representative pictures of lungs 2 weeks after the operations. Note the severe lung edema in Klf5^tm2.1Rng/Klf5^tm2.1Rng Tg(Postn-cre)1Sjc/0 mice subjected to HI-TAC. (C) Lung weights in Klf5^tm2.1Rng/Klf5^tm2.1Rng (n = 5) and Klf5^tm2.1Rng/Klf5^tm2.1Rng Tg(Postn-cre)1Sjc/0 (n = 3) mice 2 weeks after the operations. (D) Representative low-magnification views of hematoxylin and eosin (H&E)-stained heart sections 2 weeks after the operations. Scale bar: 1 mm. (E-G) Heart weight/body weight ratios (E), relative cross-sectional areas of cardiomyocytes (F), and fibrotic areas (G) in Klf5^tm2.1Rng/Klf5^tm2.1Rng (n = 5) and Klf5^tm2.1Rng/Klf5^tm2.1Rng Tg(Postn-cre)1Sjc/0 (n = 3) mice 2 weeks after the HI-TAC operation. *P < 0.01 versus sham control of the same genotype; #P < 0.05 versus Klf5^tm2.1Rng/Klf5^tm2.1Rng mice subjected to HI-TAC. (H) M-mode echocardiographic tracings obtained 2 weeks after the operations.

Copyright

This image is from Takeda N, J Clin Invest 2010 Jan;120(1):254-65 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:156696

Associated Alleles

Symbol	Name
Klf5^tm2.1Rng	Kruppel-like transcription factor 5; targeted mutation 2.1, Ryozo Nagai
Tg(Postn-cre)1Sjc	transgene insertion 1, Simon J Conway

Associated Genotypes

Allelic Composition	Genetic Background
Klf5^tm2.1Rng/Klf5^tm2.1Rng Tg(Postn-cre)1Sjc/0	involves: C57BL/6