Phenotype Image Detail

Image

Caption

Rab25 deficiency promotes intestinal adenoma formation in Apc^Min/Apc⁺ mice. (A) Gross appearance of small intestine from Apc^Min/Apc⁺ (APC^Min/+;Rab25^+/+) and Apc^Min/Apc⁺ Rab25^tm1Jrgo/Rab25^tm1Jrgo (APC^Min/+;Rab25^-/-) mice. Rab25-deficient mice showed an increase in tumor numbers and size throughout the intestine. (B) Anatomical distribution of polyps in small intestine (SI) of Apc^Min/Apc⁺ and Apc^Min/Apc⁺ Rab25^tm1Jrgo/Rab25^tm1Jrgo mice. Polyp number was determined in the proximal, middle, and distal intestine as well as in the total intestines of Apc^Min/Apc⁺ (+/+) (n = 7), Apc^Min/Apc⁺ Rab25^tm1Jrgo/Rab25⁺ (+/-) (n = 16), and Apc^Min/Apc⁺ Rab25^tm1Jrgo/Rab25^tm1Jrgo (-/-) (n = 12) mice. The distal intestine showed a greater increase in the number of polyps than the proximal intestine. Apc^Min/Apc⁺ Rab25^tm1Jrgo/Rab25⁺ mice also showed a significant increase in tumor numbers that was intermediate between Apc^Min/Apc⁺ and Apc^Min/Apc⁺ Rab25^tm1Jrgo/Rab25^tm1Jrgo mice. Data represent mean +/- SEM. (C) Representative Swiss rolls of proximal and distal intestine from Apc^Min/Apc⁺ (+/+) and Apc^Min/Apc⁺ Rab25^tm1Jrgo/Rab25^tm1Jrgo (-/-) mice. Both proximal and distal intestine from Apc^Min/Apc⁺ Rab25^tm1Jrgo/Rab25^tm1Jrgo (-/-) mice showed an increase in tumor number. Scale bars: 1 mm.

Copyright

This image is from Nam KT, J Clin Invest 2010 Mar;120(3):840-9 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:158733

Associated Alleles

Symbol	Name
Apc^Min	APC, WNT signaling pathway regulator; multiple intestinal neoplasia
Rab25^tm1Jrgo	RAB25, member RAS oncogene family; targeted mutation 1, James R Goldenring

Associated Genotypes

Allelic Composition	Genetic Background
Apc^Min/Apc^+	C57BL/6J-Apc^Min
Apc^Min/Apc^+ Rab25^tm1Jrgo/Rab25^tm1Jrgo	B6.Cg-Rab25^tm1Jrgo Apc^Min