Phenotype Image Detail

Image

Caption

(A and B) Impaired in vitro fertilization (IVF) of Pla2g3^tm1Murm/Pla2g3^tm1Murm (-/-) sperm. (A) Representative photos of wild-type (+/+) eggs incubated for 24 hours with sperm (200 cells/ul) from wild-type and mutant mice are shown. (B) Fertilization efficiency was evaluated as the number of 2-cell stage eggs (arrows in A) relative to the total number at 24 hours (mean +/- SD; n = 12~15; **P < 0.01). (C) Restored fertilization of mutant spermatozoa after removal of the zona pellucida (ZP) from eggs (mean +/- SD; n = 4; **P < 0.01). (D and E) Impaired binding of mutant sperm to ZP. Sperm from wild-type and mutant mice were incubated for 3 hours with wild-type eggs, and the nuclei of the sperm bound to the eggs were stained with Hoechst 333412. (D) Representative photos of phase-contrast (top panels) and fluorescence (bottom panels) fields are shown. (E) The numbers of spermatozoa bound to eggs were counted (mean +/- SD; n = 3; *P < 0.05). (F) Impaired motility of mutant sperm. Trails of sperm from wild-type and mutant mice were analyzed using a Sperm Motility Analysis System (original magnification, x100). (G and H) After insemination with wild-type and mutant sperm, eggs that developed to the 2-cell stage were collected on day 1 and cultured by day 3. (G) Arrows indicate eggs whose progression to the morula stage was delayed or stopped. (H) The proportion of eggs that developed into morulae relative to those at the 2-cell stage was calculated (mean +/- SD; n = 5; *P < 0.05). Scale bar: 100 um (A, G, and F); 40 um (D).

Copyright

This image is from Sato H, J Clin Invest 2010 May 3;120(5):1400-14 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:161462

Associated Alleles

Symbol	Name
Pla2g3^tm1Murm	phospholipase A2, group III; targeted mutation 1, Makoto Murakami

Associated Genotypes

Allelic Composition	Genetic Background
Pla2g3^tm1Murm/Pla2g3^tm1Murm	involves: 129S/SvEv * C57BL/6J