Phenotype Image Detail

Image

Caption

(A-F) Telomere elongation in Pinx1^tm1.1Kplu/Pinx1⁺ MEFs at late passage, as determined by qFISH. Wild-type (PinX1^+/+) and mutant heterozygote (PinX1^+/-) MEFs at early (from 3 to 5) (A-C) and late (~30) (D-F) passage were fixed and hybridized with a FITC-labeled PNA (CCCTAA)3 probe, followed by quantifying TFUs in approximately 2,000 telomeres, with a telomere distribution curve being shown in C and F. (G and H) Telomere elongation in mutant MEFs at late passage, as determined by telomere Southern blot. Multiple independent clones of wild-tye and mutant MEFs were cast into plug molds, lysed, and digested, followed by Southern blot analysis using a TTAGGG repeat as a probe. Prior to hybridization, the gels were stained with ethidium bromide to insure equal loading of total DNA, with a segment of the gels being shown in lower panels (G), with average TRF lengths from 3 independent MEF lines being quantified using ImageQuant (H). (I and J) Telomere elongation at old (10-13 months) (J), but not young age (6-10 weeks) (I) of heterozygous mutant mice, as determined by qFISH. (K and L) Telomere elongation at old, but not young age of heterozygous mutant mice, as determined by Southern blot analysis of splenocytes (K), with average TRF lengths from 5-6 littermates being quantified using ImageQuant (L). The lanes were run on the same gel, but were noncontiguous. **P < 0.01. Data are represented as mean +/- SD.

Copyright

This image is from Zhou XZ, J Clin Invest 2011 Apr 1;121(4):1266-82 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:171997

Associated Alleles

Symbol	Name
Pinx1^tm1.1Kplu	PIN2/TERF1 interacting, telomerase inhibitor 1; targeted mutation 1.1, Kun Ping Lu

Associated Genotypes

Allelic Composition	Genetic Background
Pinx1^tm1.1Kplu/Pinx1^+	involves: 129S7/SvEvBrd * C57BL/6 * FVB/N