Phenotype Image Detail

Image

Caption

(H and I) Whole-mount IHC for PECAM-1 (red) and GFP (green) in P5 (H) and P7 (I) retinas of Gt(ROSA)26Sor^{tm1(CAG-Rhoj,-EGFP)Auem}/Gt(ROSA)26Sor^{tm1(CAG-Rhoj,-EGFP)Auem} Tg(Tek-cre)#Rwng/0 (Tie2Cre;R26-Rhoj) and Gt(ROSA)26Sor^{tm1(CAG-Rhoj,-EGFP)Auem}/Gt(ROSA)26Sor^{tm1(CAG-Rhoj,-EGFP)Auem} Tg(CAG-cre/Esr1*)1Egwa/0 (MerCreMer;R26-Rhoj) mice. In I, 40HT was systemically administered to the Gt(ROSA)26Sor^{tm1(CAG-Rhoj,-EGFP)Auem}/Gt(ROSA)26Sor^{tm1(CAG-Rhoj,-EGFP)Auem} Tg(CAG-cre/Esr1*)1Egwa/0 mice at P3. Note the membrane blebbing (arrowheads) in RhoJ-overexpressing endothelial cells revealed by GFP expression at the sprouting vascular fronts. Scale bars: 20 um (H and I).

Copyright

This image is from Fukushima Y, J Clin Invest 2011 May 2;121(5):1974-85 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:173943

Associated Alleles

Symbol	Name
Gt(ROSA)26Sor^tm1(CAG-Rhoj,-EGFP)Auem	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Akiyoshi Uemura
Tg(CAG-cre/Esr1*)1Egwa	transgene insertion 1, Gyohei Egawa
Tg(Tek-cre)#Rwng	transgene insertion, Rong Wang

Associated Genotypes

Allelic Composition	Genetic Background
Gt(ROSA)26Sor^tm1(CAG-Rhoj,-EGFP)Auem/Gt(ROSA)26Sor^tm1(CAG-Rhoj,-EGFP)Auem Tg(Tek-cre)#Rwng/0	involves: C57BL/6 * CBA
Gt(ROSA)26Sor^tm1(CAG-Rhoj,-EGFP)Auem/Gt(ROSA)26Sor^tm1(CAG-Rhoj,-EGFP)Auem Tg(CAG-cre/Esr1*)1Egwa/0	involves: C57BL/6 * CBA * DBA/2