Phenotype Image Detail

Image

Caption

Reduced beta cell proliferation in obese Ghr^tm1.1Dlr/Ghr^tm1.1Dlr Tg(Ins2-cre)25Mgn/0 mice. Immunofluorescence analysis of Ki67 in pancreatic sections. Ki67 is stained green, GLUT2 red, and the nucleus blue. (B) Quantification of Ki67-positive cells per 1,000 cells based on 20-25 islets in each group. (C) Immunohistochemical staining for cyclin D2 in pancreas sections from obese control and Ghr^tm1.1Dlr/Ghr^tm1.1Dlr Tg(Ins2-cre)25Mgn/0 mice. Top and bottom rows each show representative images of the two groups. (D) Quantification of nuclear cyclin D2 staining, with determination of strong nuclear staining (Brown +++) and weak nuclear staining (Brown +). (E-I) Gene expression of cyclin D2 (Cnnd2), Gck, Irs2, Kir6.2, and Sur1 in isolated islets from obese control and mutant mice. Scale bars: 200 um. (A and C). Numbers in parentheses indicate sample size. *P < 0.05.

Copyright

This image is from Wu Y, J Clin Invest 2011 Jun 1;121(6):2422-6 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:173909

Associated Alleles

Symbol	Name
Ghr^tm1.1Dlr	growth hormone receptor; targeted mutation 1.1, Derek LeRoith
Tg(Ins2-cre)25Mgn	transgene insertion 25, Mark A Magnuson

Associated Genotypes

Allelic Composition	Genetic Background
Ghr^tm1.1Dlr/Ghr^tm1.1Dlr Tg(Ins2-cre)25Mgn/0	involves: 129S4/SvJae * C3H * C57BL/6 * DBA