Phenotype Image Detail

Image

Caption

Mtss1 deficiency affects cell motility. A) Mtss1^{Gt(CSC156)Byg}/Mtss1^{Gt(CSC156)Byg} (KO) and wild-type (WT) cells were grown in 10% serum-containing medium until confluence, then wounded by rubber policeman and incubated in medium containing either 10% or 0.2% of serum. The images showing the same wounded areas were taken at the beginning of incubation (0) and 22 h later. (B) Wound-healing rates were measured as described in the Materials and Methods. (C) Cells were arrested in 0.2% serum-containing medium and placed on the top chamber of Transwell plates in which the bottom chamber was filled with 0.2% serum medium or medium containing PDGF (30 ng/ml). The plates were incubated for 4 h, stained and photographed. (D) Quantification of the motility of cells in Transwell plates containing 0.2% serum, 30 ng/ml PDGF, 30 ng/ml EGF and 10% serum, respectively. All the presented values are the mean +/- SEM of three independent experiments.

Copyright

This image is from Yu D, PLoS One 2011;6(6):e20845, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:174444

Associated Alleles

Symbol	Name
Mtss1^Gt(CSC156)Byg	MTSS I-BAR domain containing 1; gene trap CSC156, BayGenomics

Associated Genotypes

Allelic Composition	Genetic Background
Mtss1^Gt(CSC156)Byg/Mtss1^Gt(CSC156)Byg	B6.129P2-Mtss1^Gt(CSC156)Byg