Phenotype Image Detail

Image

Caption

(A and B) Wild-type (WT) and Mapk14^tm2Nbr/Mapk14^tm2Nbr Edil3^{Tg(Sox2-cre)1Amc}/0 Mapk11^tm1Jsca/Mapk11^tm1Jsca (dKO) E13.5 embryos are shown. Dashed lines outline the liver, arrows indicate spina bifida, and the arrowhead denotes exencephaly. (C and D) BrdU staining of the neural tube sections corresponding to the areas outlined by dashed rectangles in A and B. (E) Quantification of the BrdU staining in neural tubes (three sections of each genotype). (Scale bars: 500 um.) (F and G) Liver gross morphology. (H and I) Haematoxylin and eosin-staining in liver sections. (Scale bars: 50 um.) (J) Quantification of TUNEL-positive cells in livers (three sections of each genotype). (K) Deletion of Mapk14^tm2Nbr/Mapk14^tm2Nbr (p38alphaKO) by Edil3^{Tg(Sox2-cre)1Amc} was analyzed by immunoblotting in whole lysates of E13.5 embryos. Error bars indicate SD.

Copyright

This image is from del Barco Barrantes I, Proc Natl Acad Sci U S A 2011 Aug 2;108(31):12764-9. Copyright 2011 National Academy of Sciences, U.S.A. J:176023

Associated Alleles

Symbol	Name
Edil3^Tg(Sox2-cre)1Amc	EGF-like repeats and discoidin I-like domains 3; transgene insertion 1, Andrew P McMahon
Mapk11^tm1Jsca	mitogen-activated protein kinase 11; targeted mutation 1, J Simon C Arthur
Mapk14^tm2Nbr	mitogen-activated protein kinase 14; targeted mutation 14, Angel R Nebreda

Associated Genotypes

Allelic Composition	Genetic Background
Mapk11^tm1Jsca/Mapk11^tm1Jsca Mapk14^tm2Nbr/Mapk14^tm2Nbr Edil3^Tg(Sox2-cre)1Amc/Edil3^+	involves: 129P2/OlaHsd * C57BL/6 * CBA