Phenotype Image Detail

Image

Caption

Nodal and paranodal ultrastructure in 3 and 4 week old mice. (A) Transmission electron microscopy of longitudinal sciatic nerve sections from 3 week old Sirt2^tm1.1Auw/Sirt2^tm1.1Auw Tg(Mpz-cre)26Mes/0 (Sirt2-SCKO) and Sirt2^tm1.1Auw/Sirt2^tm1.1Auw (Sirt2fl/fl) control mice. Note normal organization of the node of Ranvier with flanking paranodes in both genotypes. (Scale bar: 1 um.) (B) Normal ultrastructure of paranodal loops with properly apposed axoglial junctions (arrows) in controls (Left) and Sirt2-SCKO mice (Right) at 3 and 4 weeks of age. (Scale bar: 2 um.) (C) Representative electron micrographs showing myelin outfoldings of different size and shape (blue arrowheads) in longitudinal sciatic nerve sections from 3 and 4 week old Sirt2-SCKO mice. Note that the protrusions are located in close vicinity to normal-appearing paranodal loops (red arrows). These abnormal outfoldings were never observed in control mice. (Scale bars: 2 um.)

Copyright

This image is from Beirowski B, Proc Natl Acad Sci U S A 2011 Oct 25;108(43):E952-61. Copyright 2011 National Academy of Sciences, U.S.A. J:177468

Associated Alleles

Symbol	Name
Sirt2^tm1.1Auw	sirtuin 2; targeted mutation 1.1, Johan Auwerx
Tg(Mpz-cre)26Mes	transgene insertion 26, Albee Messing

Associated Genotypes

Allelic Composition	Genetic Background
Sirt2^tm1.1Auw/Sirt2^tm1.1Auw Tg(Mpz-cre)26Mes/0	involves: 129 * C57BL/6J * FVB/N