Phenotype Image Detail

Image

Caption

Histological analyses of mutant Ikbkap and control embryos. (A-F) H&E-stained transverse sections through the head of E9.5 wild-type (WT) (A and D), E10.5 Elp1^tm1Id/Elp1^tm1Id(B and E) and E10.5 WT (C and F). A, B, D and E are shown in the same magnification for comparison. Note that the telencephalic vesicle (tv) of the mutant embryo (E) is poorly developed compared to D and F. Note also that the lamina terminalis (lt) is absent in the mutant (B), and that there is no evidence of formation of the optic vesicle (arrowhead). In contrast, the 4th ventricle (4v) is well formed and comparable to the control embryo in A. tg = trigeminal ganglion. (G and H) H&E-stained transverse sections at the level of the otic vesicle of E9.5 WT (G) and E10.5 Elp1^tm1Id/Elp1^tm1Id (H) embryos. Note that in the E10.5 mutant the otic vesicle (ot) has separated from the overlying surface ectoderm, a landmark of normal development of E9.5 stage (G). Note that although the dorsal aorta (arrow) and cardinal vein (white arrowhead) are visible in the mutant in H they are hypoplastic compared to the embryo in G. (I-N) H&E-stained transverse sections through the heart (rostral to caudal) of E9.5 WT (I, K and M) and E10.5 Elp1^tm1Id/Elp1^tm1Id embryos (J, L and N). Note the abnormal relative position of the outflow tract (oft) in the mutant heart (L) compared to a normal embryo (K). The myocardium and also the level of trabeculation of the ventricle in the mutant (J and L) appear comparable to an E9.5 control embryo (I and K). v = ventricle, a = atrium, Li = liver. Note that development of a WT E10.5 heart has proceeded significantly (O) with extensive trabeculation, separation of left and right ventricles, and formation of the endocardial cushion (ec).

Copyright

This image is from Dietrich P, PLoS One 2011;6(10):e27015, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:178070

Associated Alleles

Symbol	Name
Elp1^tm1Id	elongator complex protein 1; targeted mutation 1, Ioannis Dragatsis

Associated Genotypes

Allelic Composition	Genetic Background
Elp1^tm1Id/Elp1^tm1Id	involves: 129S1/Sv * C57BL/6