Phenotype Image Detail

Image

Caption

A. Electron micrographs from transverse sections of phrenic nerves of 4-month-old wild-type (WT) and Epb41l3^tm1.1Jglt/Epb41l3^tm1.1Jglt (KO) mice. B. Fiber diameter, axonal diameter, and G-ratio in phrenic nerves of 4- or 13-month-old WT and KO mice. The size of the axons was slightly reduced and the thickness of the myelin sheath increased in KO mice. Statistical analysis, Mann-Whitney test, ** p<0.01, ***p<0.001 (B). C. Internodal length class histogram distribution of myelinated segments from sciatic nerves of 6-month-old WT and KO mice. Internodal lengths are less spread in KO than in WT mice, with more than 40% of the fibers with a fiber diameter of 500-600 um. Statistical analysis, Chi 2 = 27.77, p = 0.0001. D. Correlation of internodal length with nerve fiber diameter. The slopes are significantly different between the two genotypes: WT, 33.5 +/- 2.5 (r² = 0.49); KO, 21.0 +/- 3.2 (r² = 0.24). Linear regression, p<0.01. E. Visualization of Schmidt-Lanterman incisures (SLIs, NF155 labeling, in green, asterisks) and nodes (Na_v labeling, in red, arrowheads) in teased sciatic fibers from adult WT and KO mice. n, nuclei of the Schwann cells stained with DAPI (blue). F. Quantification of the average number of SLIs per mm in sciatic nerves from adult WT and KO mice. Statistical analysis, two-tailed t-test, *** p<0.0001. G. NF155 and beta-catenin expression in sciatic nerves from adult WT and KO mice evaluated by immunoblotting (data not shown), and quantified and normalized to beta-tubulin signal (three mice for each genotype). Levels of beta-catenin and NF155 are significantly increased in KO mice compared to WT mice. Unpaired t-test, *p<0.05, ** p<0.005. Scale bars: A, 10 um; E, 50 um.

Copyright

This image is from Cifuentes-Diaz C, PLoS One 2011;6(9):e25043, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:177866

Associated Alleles

Symbol	Name
Epb41l3^tm1.1Jglt	erythrocyte membrane protein band 4.1 like 3; targeted mutation 1.1, Jean-Antoine Girault

Associated Genotypes

Allelic Composition	Genetic Background
Epb41l3^tm1.1Jglt/Epb41l3^tm1.1Jglt	involves: FVB/N