Phenotype Image Detail

Image

Caption

Smad2^tm1.1Nomu/Smad2^tm1.1Nomu Tg(Nes-cre)1Kln/0 (Smad2-CNS-KO) mice cerebella and cerebrum display more TUNEL-positive cells than those of control mice. A-H, sagittal brain sections of E18.5 and P7 mice were labeled for TUNEL. At E18.5, Smad2^tm1.1Nomu/Smad2^tm1.1Nomu Tg(Nes-cre)1Kln/0 mice cerebella (A and B) and cerebrum (C and D) showed a similar number of TUNEL-positive cells per section. At P7, compared with those of control mice, mutant mice cerebella (E and F) and cerebrum (G and H) showed a higher number of TUNEL-positive cells per section. Scale bar, 200 um. Insets, high magnification images were taken from the areas indicated by rectangles. Scale bar, 50 um. Arrowheads indicate TUNEL-positive cells. I, measurement of TUNEL-positive cells in E18.5 and P7 mice. Quantification revealed that at E18.5, no significant differences were found between mutant and control mice. However, the number of apoptotic cells is significantly higher in both mutant cerebelum and cerebrum at P7. Values are expressed as the mean +/-S.D. per section of five sections per animal, three animals per group. NS, not significant. An asterisk represents p values < 0.01.

Copyright

This image is from Wang L, J Biol Chem 2011 May 27;286(21):18766-74 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:173787

Associated Alleles

Symbol	Name
Smad2^tm1.1Nomu	SMAD family member 2; targeted mutation 1.1, Masatoshi Nomura
Tg(Nes-cre)1Kln	transgene insertion 1, Rudiger Klein

Associated Genotypes

Allelic Composition	Genetic Background
Smad2^tm1.1Nomu/Smad2^tm1.1Nomu Tg(Nes-cre)1Kln/0	involves: C57BL/6 * SJL