Phenotype Image Detail

Image

Caption

A-D, staining for the presynaptic protein, synaptophysin, and DAPI were performed on P7 and P14 cerebellar sections of control and Smad2^tm1.1Nomu/Smad2^tm1.1Nomu Tg(Nes-cre)1Kln/0 (Smad2-CNS-KO) mice. Fluorescent labeling for synaptophysin represents colocalization of presynaptic terminals along the Purkinje cell dendrites. Scale bar, 50 um. E, a representative Western blot of P7 cerebella lysates probed with synaptophysin antibody (upper panel). GAPDH was used as an internal control (lower panel). F, quantitation of synaptophysin expression levels on P7 mice was performed. Error bars indicate S.D. An asterisk represents p values < 0.05.

Copyright

This image is from Wang L, J Biol Chem 2011 May 27;286(21):18766-74 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:173787

Associated Alleles

Symbol	Name
Smad2^tm1.1Nomu	SMAD family member 2; targeted mutation 1.1, Masatoshi Nomura
Tg(Nes-cre)1Kln	transgene insertion 1, Rudiger Klein

Associated Genotypes

Allelic Composition	Genetic Background
Smad2^tm1.1Nomu/Smad2^tm1.1Nomu Tg(Nes-cre)1Kln/0	involves: C57BL/6 * SJL