Phenotype Image Detail

Image

Caption

Prolonged and more severe neurological deficits after spreading depression in mutant mice. Representative photographs demonstrating the wire grip and neurological deficits after a single spreading depression (SD) in female wild-type (WT) and homozygous Cacna1a^tm1Maag/Cacna1a^tm1Maag (R192Q) and Cacna1a^tm3Maag/Cacna1a^tm3Maag (S218L) mice. In wire grip test, WT mice showed minimal deficits soon after SD (a), while Cacna1a^tm3Maag/Cacna1a^tm3Maag mice continued to fall off the wire for prolonged periods (b). Neurological examination showed leaning (c) and circling (d) in Cacna1a^tm1Maag/Cacna1a^tm1Maag and Cacna1a^tm3Maag/Cacna1a^tm3Maag mice. SD was evoked in the right hemisphere in all mice. (e) Graphs showing the time course of neurological deficits (only wire grip latency shown) from four representative knockin mice. One or more episodes of transient neurological deterioration (red arrows) were observed after full or partial recovery in 7/7 homozygous female Cacna1a^tm1Maag/Cacna1a^tm1Maag knockin, 4/6 homozygous male Cacna1a^tm1Maag/Cacna1a^tm1Maag knockin, 3/3 homozygous female Cacna1a^tm3Maag/Cacna1a^tm1Maag knockin, and 4/5 heterozygous female Cacna1a^tm3Maag/Cacna1a⁺ knockin mice. Vertical dashed line indicates a non-fatal generalized seizure that occurred shortly after the 40 min assessment time point.

Copyright

This image is from Eikermann-Haerter K, J Clin Invest 2009 Jan;119(1):99-109 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:144701

Associated Alleles

Symbol	Name
Cacna1a^tm1Maag	calcium channel, voltage-dependent, P/Q type, alpha 1A subunit; targeted mutation 1, Arn van den Maagdenberg
Cacna1a^tm3Maag	calcium channel, voltage-dependent, P/Q type, alpha 1A subunit; targeted mutation 3, Arn van den Maagdenberg

Associated Genotypes

Allelic Composition	Genetic Background
Cacna1a^tm3Maag/Cacna1a^tm3Maag	B6.129P2-Cacna1a^tm3Maag
Cacna1a^tm1Maag/Cacna1a^tm1Maag	B6.129P2-Cacna1a^tm1Maag