Phenotype Image Detail

Image

Caption

A representative renal cyst at lower (A1) and higher (A2) magnification in a newborn Lzts2^tm1.1Zsu/Lzts2^tm1.1Zsu (-/-) mouse showed upper pole renal polycystic changes (yellow arrow, A1) with hydroureter (yellow arrow, A2). Histological examination of the cystic kidneys was conducted, and the representative images showed multiple cyst formation in the parenchyma at low (B1) and high magnification (B2). Sex- and age-matched wild-type littermates were analyzed, and a representative image is shown (B3). Dolichos biflorus agglutinin (DBA, FITC-labeled) staining was performed and revealed that most of the epithelial cysts were mainly derived from the collecting system of the kidney in mutant mice (C1 and C2). An image from an age- and sex-matched wild-type littermate was shown (C3). Immunohistochemical analyses were performed with the E-cadherin antibody in the above wild-type (D1) and mutant newborns (D2 and D3). Whereas cysts lined by a single layer of epithelial cells (*) showed positive E-cadherin staining, cysts lined by epithelial cells with increased cytoplasm and englarged nuclei (**) showed significantly reduced E-cadherin staining (D2 and D3). The representative images of immunohistochemical staining with the Ki-67 antibody in the kidney of wild-type (E1) and mutant neonate kidney tissues (E2 and E3) were shown.

Copyright

This image is from Peng Y, J Biol Chem 2011 Nov 18;286(46):40331-42 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:178154

Associated Alleles

Symbol	Name
Lzts2^tm1.1Zsu	leucine zipper, putative tumor suppressor 2; targeted mutation 1.1, Zijie Sun

Associated Genotypes

Allelic Composition	Genetic Background
Lzts2^tm1.1Zsu/Lzts2^tm1.1Zsu	B6.Cg-Lzts2^tm1.1Zsu
Lzts2^tm1.1Zsu/Lzts2^+	B6.Cg-Lzts2^tm1.1Zsu