Phenotype Image Detail

Image

Caption

(A-C) Primoridal germ cells (PGCs) were visualized by wholemount SSEA1 immunostaining with SSEA1 antibody in E10.25 wild-type (WT), Ror2^Y324C/Ror2^Y324C (Ror2^Y324C), and Wnt5a^tm1Amc/Wnt5a^tm1Amc (Wnt5a-/-) embryos. (E-G) Gonadal ridges from E11.5 stained with GCNA, and (I-K) E12.5 male gonads stained with GCNA antibody. The caudal end is down in all images. (D, H, L) Quantification of PGCs in the entire E10.25 embryo, E11.5 and E12.5 gonads from confocal stacks, with individuals denoted as WT (diamonds), Ror2^Y324C/Ror2^Y324C mutants (triangle), and Wnt5a^tm1Amc/Wnt5a^tm1Amc null (circle), and means indicated as bars. Consistent with appearances, a significant reduction of PGCs was observed in Wnt5a^tm1Amc/Wnt5a^tm1Amc and from E11.5 onward in Ror2^Y324C/Ror2^Y324C mutants. We noted no significant difference in the number of PGCs between XX and XY gonads at E12.5 (not shown). Results of the Student's t-test are indicated, * p<0.05, ** p<0.01, *** p<0.001.

Copyright

This image is from Laird DJ, PLoS Genet 2011 Dec;7(12):e1002428, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:179805

Associated Alleles

Symbol	Name
Ror2^Y324C	receptor tyrosine kinase-like orphan receptor 2; Tyr324Cys
Wnt5a^tm1Amc	wingless-type MMTV integration site family, member 5A; targeted mutation 1, Andrew P McMahon

Associated Genotypes

Allelic Composition	Genetic Background
Wnt5a^tm1Amc/Wnt5a^tm1Amc	involves: 129S7/SvEvBrd
Ror2^Y324C/Ror2^Y324C	involves: C57BL/6J