Phenotype Image Detail

Image

Caption

DNA synthesis was observed by determining BrdU incorporation into cultured blastocyts in vitro. (A) Pold1^tm1Uchi/Pold1⁺ (+/-) and Pold1^tm1Uchi/Pold1^tm1Uchi (-/-) E3.5 embryos were cultured for 24 hr, then cultured in the presence of BrdU for 3 hr. After indirect immunofluorescence using anti-BrdU (green) and anti-pHH3 (red) antibodies and counterstained with DAPI (blue), embryos were genotyped by PCR. (B) E3.5 embryos were cultured for 3 days, cultured in the presence of BrdU for 3 hr, and processed for immunostaining against BrdU (green) and pHH3 (red) and DAPI counterstaining (blue). The genotype of each embryo was subsequently determined by PCR. White horizontal bar indicates 50 um.

Copyright

This image is from Uchimura A, PLoS ONE 2009;4(1):e4184, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:144992

Associated Alleles

Symbol	Name
Pold1^tm1Uchi	polymerase (DNA directed), delta 1, catalytic subunit; targeted mutation 1, Arikuni Uchimura

Associated Genotypes

Allelic Composition	Genetic Background
Pold1^tm1Uchi/Pold1^tm1Uchi	C57BL/6-Pold1^tm1Uchi
Pold1^tm1Uchi/Pold1^+	C57BL/6-Pold1^tm1Uchi