Phenotype Image Detail

Image

Caption

Islet phenotype of constitutive beta-cell glutamate dehydrogenase knock-out. A, Immunostaining of representative islets from control (left panel) and Glud1^tm1.1Pma/Glud1^tm1.1Pma Tg(Ins2-cre)23Herr/0 (BetaGlud1-/-, right panel) mice using antibodies directed against insulin to detect beta-cells (green) and against glucagon to reveal alpha-cells (red). In control animals, the majority of islets should be organized with alpha-cells surrounding the core of the structure formed by beta-cells. B, immunostaining of representative pancreatic sections from control (left panel) and mutant (right panel) mice using an antibody directed against insulin to detect beta-cells (green) and against glucagon to reveal alpha-cells (red). C, bar graph shows quantification as relative percentage of organized versus disorganized islets in control (black bars) and mutant (gray bars) pancreata. Scale bar, 50 um; n = 3 independent analyses, *, p<0.05. D, islet ATP levels following a 30-min incubation period at basal 2.8 mM (Glc 2.8) to stimulatory 22.8 mM (Glc 22.8) glucose concentrations (n = 4); *, p<0.05 versus control Glc 2.8; p<0.05 versus corresponding genotype matched Glc 2.8.

Copyright

This image is from Carobbio S, J Biol Chem 2009 Jan 9;284(2):921-9 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:145558

Associated Alleles

Symbol	Name
Glud1^tm1.1Pma	glutamate dehydrogenase 1; targeted mutation 1.1, Pierre Maechler
Tg(Ins2-cre)23Herr	transgene insertion 23, Pedro Luis Herrera

Associated Genotypes

Allelic Composition	Genetic Background
Glud1^tm1.1Pma/Glud1^tm1.1Pma Tg(Ins2-cre)23Herr/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA/J * SJL