Phenotype Image Detail

Image

Caption

Cryoimmuno electron microscopic analysis of wild-type and Cryaa^tm1Ady/Cryaa⁺ lenses. Lenses expressing either wild-type (WT) or Cryaa^tm1Ady/Cryaa⁺ were labeled with an antibody to alphaA-crystallin and analyzed by cryoimmuno electron microscopy. (A) WT lenses showed linear fiber cell membranes. Immunogold labeling (black dots) showed that alphaA-crystallin was restricted to the cytoplasm. (B) Heterozygous Cryaa^tm1Ady/Cryaa⁺ lenses had several gold particles on the fiber cell membranes. Furthermore, the linearity of lens fiber cell membranes was lost, and significant gaps between cells were apparent.

Copyright

This image is from Andley UP, BMC Ophthalmol 2009;9():4, an open-access article, licensee BioMed Central Ltd. J:157274

Associated Alleles

Symbol	Name
Cryaa^tm1Ady	crystallin, alpha A; targeted mutation 1, Usha P Andley

Associated Genotypes

Allelic Composition	Genetic Background
Cryaa^tm1Ady/Cryaa^+	involves: 129