Phenotype Image Detail

Image

Caption

A, electron microscopy images of flexor digitorum brevis (FDB) muscles from 3-month-old Tg(SOD1*G93A)1Gur/0 (G93A) and wild-type (WT) mice. In the WT, mitochondria (arrows) aligned within the sarcomeric I band and had uniform sizes (panel 2). In contrast, the mutant muscle (panel 1) had enlarged mitochondria with vacuoles (panels 3-5), invading the sarcomeric A band. B, live muscle fibers probed simultaneously with Mito-Tracker Deep Red (panels 1 and 3) and tetramethylrhodamine ethyl ester (TMRE) (panels 2 and 4). A segment with depolarized mitochondria is identified in the mutant fiber (panel 2, bracket). The corresponding segment in the MitoTracker Deep Red image shows fuzzy staining with loss of contrast, due to morphological alterations of mitochondria (panel 1). Both mitochondrial probes stain the WT fiber equally and homogeneously (panels 3 and 4). C, overlays of TMRE (green) and alpha-bungarotoxin (alpha-BTX) images (red) of dually stained mutant FDB fibers. Areas of mitochondrial defects had variable size, but they always faced the neuromuscular junction (NMJ).

Copyright

This image is from Zhou J, J Biol Chem 2010 Jan 1;285(1):705-12 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:158298

Associated Alleles

Symbol	Name
Tg(SOD1*G93A)1Gur	transgene insertion 1, Mark E Gurney

Associated Genotypes

Allelic Composition	Genetic Background
Tg(SOD1*G93A)1Gur/0	B6SJL-Tg(SOD1*G93A)1Gur/J