Phenotype Image Detail

Image

Caption

A, the response of a wild-type (WT) fiber to osmotic shock. Tetramethylrhodamine ethyl ester (TMRE) stains the entire fiber homogeneously (panel 1). Osmotic stress-induced Ca2+ release events (after panel 2) are mainly confined to the peripheral region of the fiber. B, the responses of Tg(SOD1*G93A)1Gur/0 (G93A) fibers. Lack of TMRE staining identifies fiber segments with depolarized mitochondria (panel 1). The shock-induced Ca2+ release activity was greater and not restricted to the periphery of the fiber in the segments with depolarized mitochondria (panels 3 and 4). C, evolution of fluo-4 fluorescence in regions with normal or defective mitochondria in mutant muscle fibers. Mean (+/-S.E. (error bars)) over experiments of area-averaged fluorescence, normalized to values before the osmotic shock, is shown. The difference between normal and lesioned areas was highly significant (n = 6, p < 0.0001).

Copyright

This image is from Zhou J, J Biol Chem 2010 Jan 1;285(1):705-12 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:158298

Associated Alleles

Symbol	Name
Tg(SOD1*G93A)1Gur	transgene insertion 1, Mark E Gurney

Associated Genotypes

Allelic Composition	Genetic Background
Tg(SOD1*G93A)1Gur/0	B6SJL-Tg(SOD1*G93A)1Gur/J