Phenotype Image Detail

Image

Caption

Selective depletion of Prkci from mouse podocytes causes focal segmental glomerulosclerosis. Periodic acid-Schiff staining and immunohistochemistry for nephrin and synaptopodin in Prkci^tm1Kido/Prkci^tm1.1Kido Tg(Nphs1-cre)33Mska/0 (cKO) and control kidneys at P0, P10 and P21 show that mutant mice develop segmental to global glomerulosclerosis. Boxed regions are enlarged to show an irregular pattern of nephrin staining in mutant podocytes. Loss of podocytes (arrows) and occasional adhesion of glomeruli to Bowman's capsules (arrowheads) reveal the development of focal segmental glomerulosclerosis. Consistent with massive proteinuria in mutant mice at birth, PAS staining reveals occasional hyaline droplets, representing reabsorbed urinary protein, in the proximal renal tubules at P0. Bars, 50 um.

Copyright

This image is from Hirose T, PLoS ONE 2009;4(1):e4194, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:144995

Associated Alleles

Symbol	Name
Prkci^tm1.1Kido	protein kinase C, iota; targeted mutation 1.1, Yoshiaki Kido
Prkci^tm1Kido	protein kinase C, iota; targeted mutation 1, Yoshiaki Kido
Tg(Nphs1-cre)33Mska	transgene insertion 33, Taiji Matsusaka

Associated Genotypes

Allelic Composition	Genetic Background
Prkci^tm1Kido/Prkci^tm1.1Kido Tg(Nphs1-cre)33Mska/0	involves: C57BL/6 * C57BL/6N * DBA/2