Phenotype Image Detail

Image

Caption

Ultrastructure of Prkci^tm1Kido/Prkci^tm1.1Kido Tg(Nphs1-cre)33Mska/0 podocytes. (A) Although fine foot processes and slit diaphragms are observed at P0 (arrowheads), the foot processes form irregular adhesions and slit diaphragms are apically mislocalized at P7 (arrows). Mutant podocytes at P10 demonstrates effacement of foot processes and irregular adhesions between foot processes (asterisks). Apparently, these adhesions did not form tight junctions. The glomerular basement membrane (GBM) is not significantly affected. US, urinary space; EnC, endothelial cell; CL, capillary lumen. Bars, 200 nm. (B) Immunogold electron microscopy in mutant podocytes at P7 reveals that podocalyxin (5-nm gold particles) loses its apically restricted localization, whereas ZO-1 (10-nm gold particles) localizes at the cell-cell junctions of podocytes (arrows). US, urinary space; CL, capillary lumen. Bar, 200 nm.

Copyright

This image is from Hirose T, PLoS ONE 2009;4(1):e4194, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:144995

Associated Alleles

Symbol	Name
Prkci^tm1.1Kido	protein kinase C, iota; targeted mutation 1.1, Yoshiaki Kido
Prkci^tm1Kido	protein kinase C, iota; targeted mutation 1, Yoshiaki Kido
Tg(Nphs1-cre)33Mska	transgene insertion 33, Taiji Matsusaka

Associated Genotypes

Allelic Composition	Genetic Background
Prkci^tm1Kido/Prkci^tm1.1Kido Tg(Nphs1-cre)33Mska/0	involves: C57BL/6 * C57BL/6N * DBA/2