Phenotype Image Detail

Image

Caption

A, representative photographs of subcutaneous melanoma tumors at 14 days post-implantation in wild-type (Asef +/+) and Arhgef4^tm1Taki/Arhgef4^tm1Taki (Asef -/-) mice. Scale bar, 10 mm. B, volumes of tumors developed in wild-type and mutant mice (n=11/genotype). Filled circles, wild-type; open circles, mutants. Results are expressed as the mean +/-S.E. **, p<0.01. C, tumor cross-sections were stained with anti-CD31 (brown) and counterstained with hematoxylin. Arrows indicate microvessels containing red blood cells. Scale bar, 50 um. D, immunofluorescent labeling of microvessels with anti-CD31 antibody. Scale bar, 50 um. E, quantitative analysis of vessel density within tumors from wild-type and mutant mice (n=6-7/genotype) based on CD31 staining. Open bars, wild-type; filled bars, mutants. Results are expressed as the mean +/-S.E. *, p<0.05. F, Kaplan-Meier survival curves of wild-type and mutant mice with subcutaneous melanoma (n=11/genotype).

Copyright

This image is from Kawasaki Y, J Biol Chem 2010 Jan 8;285(2):1199-207 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:159959

Associated Alleles

Symbol	Name
Arhgef4^tm1Taki	Rho guanine nucleotide exchange factor 4; targeted mutation 1, Tetsu Akiyama

Associated Genotypes

Allelic Composition	Genetic Background
Arhgef4^tm1Taki/Arhgef4^tm1Taki	involves: C57BL/6 * C57BL/6J * CBA