Phenotype Image Detail

Image

Caption

(A) Prior to TRAP staining, osteoclasts of all three genotypes were of relatively similar size. Cd68^tm1Xfen/Cd68^tm1Xfen (-/-) osteoclasts demonstrated intracellular vacuole-like structures that were not present to such an extent in CD68-expressing cells. Scale bars are 100 um. Images are representative of 3 independent experiments. (B) During the fixation process (fixative: 25 mL citrate solution [18 mM citric acid, 9 mM sodium citrate, 12 mM sodium chloride, pH 3.6], 68 mL acetone, 8 mL 37% formaldehyde), many homozygous mutant osteoclasts were reduced in size and partially detached from the culture substrate resulting in a smaller size following TRAP staining. Scale bars are 200 um. Images are representative of 3 independent experiments.

Copyright

This image is from Ashley JW, PLoS One 2011;6(10):e25838, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:178120

Associated Alleles

Symbol	Name
Cd68^tm1Xfen	CD68 antigen; targeted mutation 1, Xu Feng

Associated Genotypes

Allelic Composition	Genetic Background
Cd68^tm1Xfen/Cd68^tm1Xfen	C57BL/6J-Cd68^tm1Xfen