Phenotype Image Detail

Image

Caption

Ptk2^tm1.1Itl/Ptk2^tm1.1Itl (FAK^R454/R454) is embryonic lethal with vascular defects. A, bright field images of excised yolk sacs with embryos reveals a lack of blood-filled vessels on Ptk2^tm1.1Itl/Ptk2^tm1.1Itl embryos compared with wild-type (FAK^WT/WT) embryos at E9.5. The red color within the mutant yolk sac (arrow) is associated with internal embryo hemorrhage. Bar, 100 um. B, at E9.5, wild-type embryos have undergone turning and have a defined head, beating heart, and multiple segmental somites (arrows). Mutant embryos at E9.5 have undergone turning, are small, and possess head and heart structures that are malformed (arrows). No somites or beating hearts were detectable in mutant embryos. The allantois is enlarged, does not undergo chorion fusion, and is frequently a site of hemorrhage within mutant embryos. Bar, 100 um. C, whole mount anti-CD31 staining of yolk sacs at E8.5 reveals equivalent vascular plexus formation in mutant and wild-type embryos. At E9.5, ECs within yolk sacs of wild-type embryos form a primary capillary plexus characterized by tubules and branched structures (arrowheads). The primary capillary plexus is not formed in mutant yolk sacs at E9.5 because this remains a vascular plexus structure. Bar, 20 um.

Copyright

This image is from Lim ST, J Biol Chem 2010 Jul 9;285(28):21526-36 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:165360

Associated Alleles

Symbol	Name
Ptk2^tm1.1Itl	PTK2 protein tyrosine kinase 2; targeted mutation 1.1, inGenious Targeting Laboratory

Associated Genotypes

Allelic Composition	Genetic Background
Ptk2^tm1.1Itl/Ptk2^tm1.1Itl	involves: 129S/SvEv * C57BL/6 * SJL