Phenotype Image Detail

Image

Caption

Alizarin red/alcian blue staining of the skeleton in the 1-week-old Fam20c^tm1.1Cqi/Fam20c^tm1.1Cqi Edil3^{Tg(Sox2-cre)1Amc}/0 mice. (A) The mutant mice (upper) showed smaller size and remarkably delayed ossification (more blue staining) in all bones (arrowheads) when compared with wild-type (WT) littermates (lower). (B) The rib cartilages of mutant mice (upper) stained blue, while those of their WT littermates (lower) showed broad areas with the red staining indicating ossification (arrowheads) suggesting an aberrant endochondral ossification in the mutant mice. (C) The femur of mutant mice (left) had shorter length and delayed secondary ossification center (arrow, blue stained), in contrast with the red secondary ossification center of their WT littermates (right). (D) The ossification centers in the carpus of the mutant mice (left) had more blue-stained areas than that of the WT littermates (right). (E) The skull of the mutant mice (left) had smaller size and delayed suture closure (arrows), when compared with the WT littermates (right), indicating that the intramembranous ossification was disturbed in the mutant mice. Scale bars: 1 cm in A, 2 mm in E, 1 mm in B-D.

Copyright

This image is from Wang X, PLoS Genet 2012 May;8(5):e1002708, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:185208

Associated Alleles

Symbol	Name
Edil3^Tg(Sox2-cre)1Amc	EGF-like repeats and discoidin I-like domains 3; transgene insertion 1, Andrew P McMahon
Fam20c^tm1.1Cqi	FAM20C, golgi associated secretory pathway kinase; targeted mutation 1.1, Chunlin Qin

Associated Genotypes

Allelic Composition	Genetic Background
Fam20c^tm1.1Cqi/Fam20c^tm1.1Cqi Edil3^Tg(Sox2-cre)1Amc/Edil3^+	involves: 129S6/SvEvTac * C57BL/6 * CBA