Phenotype Image Detail

Image

Caption

(A) Goldner-Masson trichrome staining of sagittal sections from the undecalcified tibias of the Fam20c^tm1.1Cqi/Fam20c^tm1.1Cqi Edil3^{Tg(Sox2-cre)1Amc}/0 (cKO) mice (left) and their wild-type (WT) littermates (right). The mutant mice showed more red staining (unmineralized osteoid) and less green staining (mineralized bone) compared with the WT. (B) Higher magnification of the cortical bone areas in A showed that the cortical bone of the mutant mice (upper) had more osteoid (red stained areas indicated by arrowheads), compared to the WT (lower). (C) IHC staining against biglycan on the sagittal sections of tibias from 3-week-old mutant mice (left) and their WT littermates (right). Note that the mutant mice had more biglycan. (D) Higher magnification of the cortical bone areas in C showed that the cortical bone of mutant mice (upper) had more biglycan (arrowheads), compared with the WT (lower). (E) Double fluorescence labeling of the tibia from 6-week-old mutant mice (left) and WT littermates (right). The first injection (calcein) produced a green label, while the second injection (Alizarin Red) gave rise to a red label. The distance between the green and red labeling indicated the mineral deposition in the period between the two injections (7 days). The tibia cortical bone of the mutant mice (left) showed narrower distance and blurry boundary between the two labels compared with the WT (right). (F) The quantitative measurements of the distance between the two injections revealed a significantly lower mineral deposition rate in the mutant mice compared with their WT littermates. *** P<0.005. Scale bars: 200 um in A and C, 50 um in B, D and E.

Copyright

This image is from Wang X, PLoS Genet 2012 May;8(5):e1002708, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:185208

Associated Alleles

Symbol	Name
Edil3^Tg(Sox2-cre)1Amc	EGF-like repeats and discoidin I-like domains 3; transgene insertion 1, Andrew P McMahon
Fam20c^tm1.1Cqi	FAM20C, golgi associated secretory pathway kinase; targeted mutation 1.1, Chunlin Qin

Associated Genotypes

Allelic Composition	Genetic Background
Fam20c^tm1.1Cqi/Fam20c^tm1.1Cqi Edil3^Tg(Sox2-cre)1Amc/Edil3^+	involves: 129S6/SvEvTac * C57BL/6 * CBA