Phenotype Image Detail

Image

Caption

Growth plate defects in 3-week-old Fam20c^tm1.1Cqi/Fam20c^tm1.1Cqi Edil3^{Tg(Sox2-cre)1Amc}/0 mice. (A) In situ hybridization (ISH) staining of collagen II (Col II) on the sagittal sections of tibia showed less Col II in the growth plate of Fam20c^tm1.1Cqi/Fam20c^tm1.1Cqi Edil3^{Tg(Sox2-cre)1Amc}/0 (cKO) mice. The pink/red color indicates positive ISH staining. (B) Higher magnification of the boxed area in A revealed less Col II in both the proliferative chondrocytes (dashed lines) and hypertrophic chondrocytes (solid lines) in the growth plate of the mutant mice compared with wild-type (WT) littermates. (C) ISH staining of collagen X (Col X) showed lower expression in the growth plate of the mutant mice. (D) Higher magnification of the boxed area in C revealed less Col X in the hypertrophic chondrocytes (solid lines) of the mutant mice compared with WT. (E) BrdU staining on the sagittal sections of tibia. (F) Higher magnification of the boxed area in E revealed fewer proliferating cells in the zone of proliferative chondrocytes (dashed lines) of the mutant mice compared with WT. (G) TUNEL assay revealed fewer apoptotic cells in the zone of hypertrophic chondrocytes (arrowheads) of the mutant mice (left) compared with WT littermates (right). Counter stained with DAPI. (H) Statistical counting of apoptotic cells in G showed significantly less number in the growth plate of the mutant mice. ***P value<0.001. Scale bars: 200 um in A, C, E and G. 50 um in B, D and F.

Copyright

This image is from Wang X, PLoS Genet 2012 May;8(5):e1002708, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:185208

Associated Alleles

Symbol	Name
Edil3^Tg(Sox2-cre)1Amc	EGF-like repeats and discoidin I-like domains 3; transgene insertion 1, Andrew P McMahon
Fam20c^tm1.1Cqi	FAM20C, golgi associated secretory pathway kinase; targeted mutation 1.1, Chunlin Qin

Associated Genotypes

Allelic Composition	Genetic Background
Fam20c^tm1.1Cqi/Fam20c^tm1.1Cqi Edil3^Tg(Sox2-cre)1Amc/Edil3^+	involves: 129S6/SvEvTac * C57BL/6 * CBA