Phenotype Image Detail

Image

Caption

Bmi1 cooperates with Pten haploinsufficiency in prostate cancer progression. (D-G) H&E stained prostate sections. (D) Normal epithelium in Tg(Pbsn-cre)4Prb/0 (PbCre4) controls. (E) Prostatic intraepithelial neoplasia (PIN) lesion (asterisk) in Tg(Pbsn-cre)4Prb/0 Gt(ROSA)26Sor^{tm1(CAG-Bmi1,-EGFP)Nki}/Gt(ROSA)26Sor⁺ (PbCre4;Bmi1^LSL) mice. (F) PIN lesion (asterisk) in Tg(Pbsn-cre)4Prb/0 Pten^tm1Mro/Pten⁺ (PbCre4;Pten^loxP/+) mice. (G) Adenocarcinoma in Tg(Pbsn-cre)4Prb/0 Gt(ROSA)26Sor^{tm1(CAG-Bmi1,-EGFP)Nki}/Gt(ROSA)26Sor⁺ Pten^tm1Mro/Pten⁺ (PbCre4;Bmi1^LSL;Pten^loxP/+) animals. Black arrowhead, mitotic figure; white arrowheads, blood vessels. Insets are enlarged 5-fold. (H-O) Immunohistochemical expression analysis of Bmi1-phosphorylated Akt (H-K) and pAkt (S473) (L-O). Scale bars: 100 um.

Copyright

This image is from Nacerddine K, J Clin Invest 2012 May 1;122(5):1920-32 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:184533

Associated Alleles

Symbol	Name
Gt(ROSA)26Sor^tm1(CAG-Bmi1,-EGFP)Nki	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, The Netherlands Cancer Institute
Pten^tm1Mro	phosphatase and tensin homolog; targeted mutation 1, Silvia Marino
Tg(Pbsn-cre)4Prb	transgene insertion 4, Pradip Roy-Burman

Associated Genotypes

Allelic Composition	Genetic Background
Gt(ROSA)26Sor^tm1(CAG-Bmi1,-EGFP)Nki/Gt(ROSA)26Sor^+ Tg(Pbsn-cre)4Prb/0	involves: 129P2/OlaHsd * C57BL/6 * DBA/2 * FVB/N
Gt(ROSA)26Sor^tm1(CAG-Bmi1,-EGFP)Nki/Gt(ROSA)26Sor^+ Pten^tm1Mro/Pten^+ Tg(Pbsn-cre)4Prb/0	involves: 129P2/OlaHsd * C57BL/6 * DBA/2 * FVB/N
Pten^tm1Mro/Pten^+ Tg(Pbsn-cre)4Prb/0	involves: C57BL/6 * DBA/2