Phenotype Image Detail

Image

Caption

Histological analysis of testis sections reveals disruption to the seminiferous epithelium associated with a reduction in numbers of post-meiotic germ cells. (a) Testes from wild-type (+/+) and Katnal1^1H/Katnal1^1H animals appear similar at day 22 when examined by light microscopy. However, at day 35 disruption to the seminiferous epithelium is apparent in mutant testes, consistent with a significant reduction in seminiferous tubule diameter. At day 70, the seminiferous epithelium in the mutant testis appears disorganised and elongate spermatids (arrowed in WT) are almost absent. (b) The apparent reduction in elongate spermatid numbers was confirmed (arrows) using an antibody against PGK1/2 which marks post-meiotic germ cells from step 8. (c) The reduction in elongate spermatids was associated with a clear reduction in apical ectoplasmic specialisations (marked by Espin) in mutant testes (arrows in +/+). (+/+ = Wild-type; neg = no-primary control; Bar = 100 um unless stated).

Copyright

This image is from Smith LB, PLoS Genet 2012 May;8(5):e1002697, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:185195

Associated Alleles

Symbol	Name
Katnal1^1H	katanin p60 subunit A-like 1; ENU mutation 1, Harwell

Associated Genotypes

Allelic Composition	Genetic Background
Katnal1^1H/Katnal1^1H	involves: C3H/HeH * C57BL/6J