Phenotype Image Detail

Image

Caption

Loss of KATNAL1 function leads to reduced numbers of stable microtubules in Sertoli cells. (a) Western blot analysis and (b) quantification of stable (glu) and dynamic (tyr) alpha-tubulin on day 22 testes from wild-type (+/+) and homozygous Katnal1^1H/Katnal1^1H animals reveals a significant reduction in numbers of stable microtubules in testes from mutants (n=5-7). (c) Immunohistochemical localisation of glu-alpha-tubulin on corresponding day 22 testis sections localises stable microtubules to Sertoli cell cytoplasm (arrows) and developing spermatids and in wild-type animals. Conversely stable microtubules are below detection limits in much of the Sertoli cell cytoplasm in homozygous mutant testes. (WT1 = Wilms Tumour 1, SC-specific loading control).

Copyright

This image is from Smith LB, PLoS Genet 2012 May;8(5):e1002697, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:185195

Associated Alleles

Symbol	Name
Katnal1^1H	katanin p60 subunit A-like 1; ENU mutation 1, Harwell

Associated Genotypes

Allelic Composition	Genetic Background
Katnal1^1H/Katnal1^1H	involves: C3H/HeH * C57BL/6J