Phenotype Image Detail

Image

Caption

Mirc21 (miR-34bc) regulates osteoblast differentiation. (A) Mineralized nodule formation of Mirc21^tm1.1Kry/Mirc21^tm1.1Kry (miR-34bc fl/fl) control and Mirc21^tm1.1Kry/Mirc21^tm1.1Kry Tg(Col1a1-cre)1Kry/0 (miR-3bc^Osb-/-) osteoblasts after 2 weeks of differentiation ex vivo, determined by Von Kossa staining and quantified by ImageJ (n = 3). (B) Expression of Mirc21 in tibiae of E10.5, E14.5, E15.5, and E16.5 embryos. (C and D) Alcian blue/alizarin red staining of skeletal preparations of Mirc21^tm1.1Kry/Mirc21^tm1.1Kry Tg(Col1a1-cre)1Kry/0 embryos. Arrowheads point to regions of premature ECM mineralization in the mutant embryos. The area of mineralization over tissue area was measured using ImageJ. (F) Expression of Osteocalcin, Bsp, and Osterix in calvaria of E14.5 and E16.5 mutant embryos (n = 3-5). Error bars indicate means +/- standard errors of the mean. *, P < 0.05. Bar, 100 um.

Copyright

This image is from Wei J, J Cell Biol 2012 May 14;197(4):509-21, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:185130

Associated Alleles

Symbol	Name
Mirc21^tm1.1Kry	microRNA cluster 21, including Mir34c through Mir34b; targeted mutation 1.1, Gerard Karsentry
Tg(Col1a1-cre)1Kry	transgene insertion 1, Gerard Karsenty

Associated Genotypes

Allelic Composition	Genetic Background
Mirc21^tm1.1Kry/Mirc21^tm1.1Kry Tg(Col1a1-cre)1Kry/0	involves: 129S4/SvJaeSor * 129S6/SvEvTac * FVB/N