Phenotype Image Detail

Image

Caption

Defective migration of granule cells in Trio^tm1Mzhu/Trio^tm1Mzhu Tg(Nes-cre)1Kln/0 (Trio^NKO) cerebella in vivo and in vitro. A, dividing granule cells of the cerebella were pulse-labeled at P9 by BrdUrd and then visualized by BrdUrd immunofluorescent staining at indicated times after injection. B, microexplants of P4 external granule cell layer (EGL) tissues 36 h after culture. Disordered migration of granule cells occurred in the mutant explant. C, decrease in mean migration distance of granule cells from the edge of aggregates in the Trio mutant; **, p < 0.01. D, microexplants were prepared from P4 EGL and cultured for 30 h before imaging. Explants are at the bottom of the frame. Intervals between pictures were 50 min. Control granule cells migrated in the opposite direction to the explant (arrows and arrowheads), whereas most Trio-deficient granule cells moved randomly (arrows) or backwards to the explant (asterisk). Scale bars: A, 25 um; B, 100 um; and D, 50 um.

Copyright

This image is from Peng YJ, J Biol Chem 2010 Aug 6;285(32):24834-44 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:165902

Associated Alleles

Symbol	Name
Tg(Nes-cre)1Kln	transgene insertion 1, Rudiger Klein
Trio^tm1Mzhu	triple functional domain (PTPRF interacting); targeted mutation 1, Min-Sheng Zhu

Associated Genotypes

Allelic Composition	Genetic Background
Tg(Nes-cre)1Kln/0 Trio^tm1Mzhu/Trio^tm1Mzhu	involves: 129S6/SvEvTac * C57BL/6 * SJL