Phenotype Image Detail

Image

Caption

A, histological analysis of E18.5 Tg(Prrx1-cre)1Cjt/0 (Prx1-Cre/Vcan+/+; control) (a and c) and Vcan^tm1.1Hwat/Vcan^tm1.1Hwat Tg(Prrx1-cre)1Cjt/0 (Prx1-Cre/Vcan^flox/flox; mutant) (b and d) hind limbs by hematoxylin and eosin staining. Control digits demonstrate a well organized columnar structure of prehypertrophic chondrocytes with vascular invasion in metatarsus (m) and proximal phalanges (pp), whereas mutant digits exhibit formation of hypertrophic chondrocyte nodules in a concentric pattern in proximal phalanges (pp) (arrows in b) and delayed endochondral ossification of metatarsus (d). In addition, clefting of some metatarsophalangeal joints is observed (b). Scale bars, 100 um (a and b) and 40 um (c and d). B, histological analysis of E15.5 control and mutant hind limbs by hematoxylin/eosin and Alcian blue staining. Control digits display horizontal stripes of the metatarsophalangeal joint interzones (a and c (as an enlarged image of the boxed area in a)). Control metatarsus shows prehypertrophic and hypertrophic chondrocyte layers (e). In contrast,mutant digits display tilted joint interzones (b and d (as an enlarged image of the boxed area in b)). Mutant metatarsus shows prehypertrophic chondrocytes but not hypertrophic chondrocytes (f). Alcian blue staining of control hind limb shows well aligned stripes of the interzone (g), whereas that of mutant digits reveals tilting of the joint interzone (indicated by arrowheads in h). Scale bars, 150 um (a and b), 30 um (c and d), 50 um (e and f), and 70 um (g and h). The number of embryos analyzed was as follows: n = 2 each at E18.5; n = 3 and 5 for mutant and control at E15.5, respectively). At least two paraffin-embedded blocks were obtained, and their tissue sections were used for histological analysis.

Copyright

This image is from Choocheep K, J Biol Chem 2010 Jul 2;285(27):21114-25 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:165936

Associated Alleles

Symbol	Name
Tg(Prrx1-cre)1Cjt	transgene insertion 1, Clifford J Tabin
Vcan^tm1.1Hwat	versican; targeted mutation 1.1, Hideto Watanabe

Associated Genotypes

Allelic Composition	Genetic Background
Vcan^tm1.1Hwat/Vcan^tm1.1Hwat Tg(Prrx1-cre)1Cjt/0	involves: C57BL/6 * C57BL/6J * SJL/J