Phenotype Image Detail

Image

Caption

A, immunostaining for versican of E15.5 Tg(Prrx1-cre)1Cjt/0 (Prx1-Cre/Vcan+/+; control) and Vcan^tm1.1Hwat/Vcan^tm1.1Hwat Tg(Prrx1-cre)1Cjt/0 (Prx1-Cre/Vcan^flox/flox; mutant) hind limbs. In control digits, versican is strongly immunostained in the interzone and perichondrium and moderately immunostained in the proliferative zone of cartilage (a and c), whereas in mutant digits, versican is immunostained neither in the interzone, the perichondrium, nor the proliferative zone of cartilage (b and d). Scale bars, 120 um (a and b) and 70 um (c and d). B, immunostaining for Cre enzyme of E15.5 mutant hind limbs at low (top) and high (bottom) magnifications. Cre enzyme is immunostained in the perichondrium and in the tilted interzone. Scale bars, 100 and 50 um, respectively. Two individual mutant digits showed the same abnormalities.

Copyright

This image is from Choocheep K, J Biol Chem 2010 Jul 2;285(27):21114-25 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:165936

Associated Alleles

Symbol	Name
Tg(Prrx1-cre)1Cjt	transgene insertion 1, Clifford J Tabin
Vcan^tm1.1Hwat	versican; targeted mutation 1.1, Hideto Watanabe

Associated Genotypes

Allelic Composition	Genetic Background
Vcan^tm1.1Hwat/Vcan^tm1.1Hwat Tg(Prrx1-cre)1Cjt/0	involves: C57BL/6 * C57BL/6J * SJL/J