Phenotype Image Detail

Image

Caption

Neuromuscular synapse analysis. A, double labeling of P0 diaphragm muscles of wild-type (+/+) and App^tm3.1Zhe/App^tm3.1Zhe (APP/hAbeta/mutC ki/ki) littermates with the anti-choline transporter (CHT) antibody and alpha-bungarotoxin that recognizes the postsynaptic acetylcholine receptors (AchR). Merge, overlay of CHT and AchR images. The open arrow marks the CHT staining beyond the end plates, and the arrowheads label the synaptic sites with sparse CHT staining. B, quantification of the percentage of AchR-positive endplates covered by CHT immunoreactivity (average +/- S.E. of 20 endplates per genotype). C, whole-mount staining of P0 diaphragm muscles of App^tm3.1Zhe/App^tm3.1Zhe Aplp2^tm1Dbo/Aplp2^tm1Dbo (App^ki/kiAPLP2-/-) mutants (ki/ki) and littermate Aplp2^tm1Dbo/Aplp2^tm1Dbo (APP+/+APLP2-/-) controls (ctrl) with an anti-synaptophysin (Syn) antibody and alpha-bungarotoxin (AchR), showing diffused pre- and postsynaptic distribution in the ki/ki mutant. Merge, overlay of Syn and AchR images. D, quantification of the average bandwidth of AchR-positive endplates. E, higher magnification images of synapse structures showing axonal staining of Syn and poorly covered endplates by Syn and extrasynaptic Syn staining in the ki/ki mutant. Merge, overlay of Syn and AchR images. F, quantification of the percentage of AchR-positive endplates covered by Syn (average +/- S.E. of 20 endplates/genotype). ***,p < 0.001;*,p < 0.05 (Student's t test). Scale bars in A and E, 20 um; scale bar in C, 100 um.

Copyright

This image is from Li H, J Biol Chem 2010 Oct 1;285(40):30598-605 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:166379

Associated Alleles

Symbol	Name
Aplp2^tm1Dbo	amyloid beta precursor-like protein 2; targeted mutation 1, David R Borchelt
App^tm3.1Zhe	amyloid beta precursor protein; targeted mutation 3.1, Hui Zheng

Associated Genotypes

Allelic Composition	Genetic Background
Aplp2^tm1Dbo/Aplp2^tm1Dbo App^tm3.1Zhe/App^tm3.1Zhe	involves: 129/Sv * 129X1/SvJ * C57BL/6