Phenotype Image Detail

Image

Caption

Vestibular hair cell planar polarity phenotypes are enhanced in Vangl2^tm1.2Mdea/Vangl2^Lp (vangl2^deltaTMs/Lp) utricle relative to Vangl2^tm1.2Mdea/Vangl2^tm1.2Mdea (vangl2^{deltaTMs/deltaTMs}). (A) A polarized bundle of stereocilia is located adjacent to the kinocilium on the apical surface of vestibular hair cells. As indicated by annotated gray arrows, movements of the bundle towards the kinocilium produce excitatory electrophysiological responses while movements away are inhibitory. (B) Planar polarity is visualized by labeling the stereocilia with phalloidin (green) and the basal body beneath the kinocilium with anti-pericentrin antibodies (red). Pericentrin labeling also marks the position of a primary cilium on the intervening supporting cells which lack stereocilia. White arrows highlight single basal bodies in hair cells and arrowheads show single basal bodies in supporting cells. (C) Schematic illustration of the range of hair cell polarities (gray arrows), their organization about the line of polarity reversal (LPR, red dashed line), and the position of three analysis fields used throughout this study. (D) Wild-type utricle hair cells labeled for planar polarity analysis with phalloidin (green) and pericentrin antibodies (red), and the position of the three analysis fields. (D9) All samples are also labeled for Gata3 (blue) to mark the striola region for analysis field placement. For illustration purposes, individual hair cell orientations have been annotated based upon the labeling in (D) and have been superimposed on the Gata3 micrograph (D9). (E,E9) Utricles from Vangl2^tm1.2Mdea/Vangl2^tm1.2Mdea mice have misoriented hair cells that are restricted to analysis field2. (F,F9) Utricles from Vangl2^tm1.2Mdea/Vangl2^Lp mice have misoriented hair cells throughout fields 1&2. Scale bar for (B) is 10 um. The dimensions of the analysis fields are all 100 um x 50 um.

Copyright

This image is from Yin H, PLoS One 2012;7(2):e31988, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:185311

Associated Alleles

Symbol	Name
Vangl2^Lp	VANGL planar cell polarity 2; loop tail
Vangl2^tm1.2Mdea	VANGL planar cell polarity 2; targeted mutation 1.2, Michael R Deans

Associated Genotypes

Allelic Composition	Genetic Background
Vangl2^Lp/Vangl2^tm1.2Mdea	involves: 129S/Sv * C57BL/6 * FVB/N * LPT/LeJ * SJL
Vangl2^tm1.2Mdea/Vangl2^tm1.2Mdea	B6NCrl.129S6(Cg)-Vangl2^tm1.2Mdea